Identification of DNA fragments carrying ecotropic proviruses of AKR mice.
نویسندگان
چکیده
منابع مشابه
Identification of DNA fragments carrying ecotropic proviruses of AKR mice.
The proviruses of the N-tropic, ecotropic virus (AKV) of AKR mice (Akv-1, Akv-2) have been studied by the Southern gel--filter transfer technique. These proviruses can be detected by cleavage of cell DNA by BamHI endonuclease, which yields characteristic subgenomic DNA fragments upon cleavage of this type of provirus. Proviruses integrated into different sites in the mouse genome can be resolve...
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AKR mice, which produce high titers of ecotropic virus, were crossed with NZB mice, which produce titers of xenotropic virus. Spleen, marrow, and lymph node cells of the F1 hybrid produced high titers of ecotropic and xenotropic viruses. However, expression of ecotropic virus by both thymus cells and peripheral T cells of the F1 was severely restricted. Despite simultaneous expression of ecotor...
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Polytropic murine leukemia viruses (MuLVs) are generated by recombination of ecotropic MuLVs with env genes of a family of endogenous proviruses in mice, resulting in viruses with an expanded host range and greater virulence. Inbred mouse strains contain numerous endogenous proviruses that are potential donors of the env gene sequences of polytropic MuLVs; however, the precise identification of...
متن کاملBrief Definitive Report RESTRICTED EXPRESSION OF ECOTROPIC VIRUS BY THYMOCYTES OF LEUKEMIA-RESISTANT (AKR × NZB)F1 MICE*
The thymus has a central role in the pathogenesis of l eukemia in AKR mice. Thymectomy great ly reduces the incidence of l eukemia (1). Moreover, v i r tua l ly all t issues of AKR mice express high t i ters of Gross (ecotropic, N-tropic) virus (2), but neoplasms develop regular ly only in the thymus. The thymus of the AKR mouse is also the site of two impor tant virological changes tha t occur...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1979
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.76.9.4554